To Determine the effect of Increasing Enzyme Concentration on the Rate of Enzyme Catalysed Reaction

Lab#:6

Title: Enzymes

Aim: To determine the effect of increasing enzyme concentration on the rate of an enzyme catalysed reaction.

Apparatus and Materials: Potato extract, hydrogen peroxide, distilled water,measuring cylinder, ruler, beakers and timer.

Diagram:

Method:

1.      Label four beakers A,B,C and D respectively.

2.      Use the data in table below to prepare the respective mixtures for each of the above labeled beakers: (I do not want to see the table below in your lab books, you must write out each step described in the table below!!!!)

Beaker	Volume of Potato Extract	Volume of Hydrogen peroxide	Volume of Distilled water
A	25ml	15ml	0ml
B	20ml	15ml	5ml
C	15ml	15ml	10ml
D	10ml	15ml	15ml

3.      Allow each beaker to stand for three minutes , then measure the bubbles produced.

4.      Calculate the rate of reaction for each beaker.

5.      Record your results in a suitable table.

6.      Use your results to plot an appropriate graph.

Calclulations :

Discussion questions:

1.      Define enzymes.

2.      Account for your results.

3.      List two limitations of the experiment.