To Determine the effect of Increasing Enzyme Concentration on the Rate of Enzyme Catalysed Reaction

Lab#:6

Title: Enzymes

Aim: To determine the effect of increasing enzyme concentration on the rate of an enzyme catalysed reaction.

Apparatus and Materials: Potato extract, hydrogen peroxide, distilled water,measuring cylinder, ruler, beakers and timer.

Diagram:

Method:

1.      Label four beakers A,B,C and D respectively.

2.      Use the data in table below to prepare the respective mixtures for each of the above labeled beakers: (I do not want to see the table below in your lab books, you must write out each step described in the table below!!!!)

Beaker	Volume of Potato Extract	Volume of Hydrogen peroxide	Volume of Distilled water
A	25ml	15ml	0ml
B	20ml	15ml	5ml
C	15ml	15ml	10ml
D	10ml	15ml	15ml

3.      Allow each beaker to stand for three minutes , then measure the bubbles produced.

4.      Calculate the rate of reaction for each beaker.

5.      Record your results in a suitable table.

6.      Use your results to plot an appropriate graph.

Calclulations :

Discussion questions:

1.      Define enzymes.

2.      Account for your results.

3.      List two limitations of the experiment.

To Determine an Enzyme's Optimal Temperature

Lab#:

Title: Enzymes

Aim: To determine the optimal temperature of an enzyme.

Apparatus & Materials:

Diagram:

Method:

1.      Pour 10ml of potato extract in a clean, dry beaker and place beaker in water bath that is at 50⁰C.

2.      Immediately add 10ml of hydrogen peroxide to beaker with potato extract.

3.      Remove the beaker at the end of three minutes and use a ruler to measure the height of bubbles produced.

4.      Repeat the above steps at 40⁰C, 35^OC, 32⁰C , 20⁰C and 10⁰C.

5.      Use an appropriate equation to calculate the rate of reaction in m/s (standard form please)

6.      Record your results in a suitable table.

7.      Use your results to plot an appropriate graph.

Calculations:

Discussion questions:

1.      Define enzyme, optimum temperature and rate of reaction.

2.      State the importance of hydrogen peroxide degradation.

3.      Account for your results briefly. Identify the enzyme, substrates and products (write a chemical equation that summarizes the reaction). What factors were kept constant and why were they kept constant?

4.      Why did you blend potato?

5.      List two limitations.